Alternatively, S. putrefaciens APR-246 UndA could function as an interchangeable module of MtrC in its interaction with other components in respiratory electron transfer reactions . S. putrefaciens undA has no obvious orthologs in most Shewanella strains including S. oneidensis MR-1. Because comparative genomic analysis
has revealed that UndA substitutes for OmcA in a number of Shewanella species [13, 33], it is possible that UndA has a IPI-549 similar function as OmcA. However, our findings argued against this possibility, as mutant phenotypes of S. oneidensis OmcA differed substantially from those of W3-18-1 UndA in that S. oneidensis OmcA was important for Fe2O3 reduction and no linkage between OmcA and MtrC was detected under ferric citrate-reducing condition . Rather, we noted that S. oneidensis ΔmtrF mutant displayed similar phenotypes as what were observed in our S. putrefaciens ΔundA mutant. It caused no deficiency of iron reduction, but progressively slower iron reduction in the absence of S. oneidensis MtrC . These results suggested that S. oneidensis MtrF might function similarly as S. putrefaciens UndA. In support of this view, the overall structural fold of UndA is significantly similar to that of MtrF, despite low protein sequence identity [32, 35]. Conclusions Comparative
genomic studies have provided important clues into the gene diversity in the respiratory systems. Combining it with experimental studies brings us closer to understand selleck chemical the genetic variations
of Shewanella genus. Using these approaches, we show in this study that UndA has a functional relatedness to MtrF, and MtrC and UndA play primary and auxiliary roles in iron reduction of W-3-18-1, respectively. Acknowledgement This research was supported by grants to Yunfeng Yang from National Science Foundation of China (41171201) and National Key Basic Research Program of China (2013CB956601), to Jizhong Zhou by The United States Department of Energy’s Office of Biological and Environmental Research under the Genomics: GTL Program through the Shewanella Federation, and the Microbial Genome Program. Electronic supplementary material Additional Reverse transcriptase file 1: Supplemental tables and figures associated with this manuscript. (DOCX 7 MB) References 1. Shi L, Squier TC, Zachara JM, Fredrickson JK: Respiration of metal (hydr) oxides by Shewanella and Geobacter: a key role for multihaem c‒type cytochromes. Mol Microbiol 2007,65(1):12–20.PubMedCrossRef 2. Tiedje JM: Shewanella—the environmentally versatile genome. Nat Biotechnol 2002,20(11):1093–1094.PubMedCrossRef 3. Viamajala S, Peyton BM, Sani RK, Apel WA, Petersen JN: Toxic effects of chromium (VI) on anaerobic and aerobic growth of shewanella oneidensis MR‒1. Biotechnol Progr 2004,20(1):87–95.CrossRef 4. Lovley DR, Holmes DE, Nevin KP: Dissimilatory Fe(III) and Mn(IV) reduction. Adv Microb Physiol 2004, 49:219–286.PubMedCrossRef 5.