54 It has been demonstrated that various cellular events including self-renewal and tumorigenicity
of cancer stem cells could be regulated by miRNAs.55 Previous studies have elucidated that TGF-β signaling promotes the transaction of primary miRNAs to precursor miRNAs and facilitates miRNA maturation by way of Smad/Drosha-dependent machinery.56 Of note, we showed that miR-216a instead of miR-21, which was reported to regulate PTEN expression in human HCC,30 was involved in PTEN suppression and hepatic T-ICs generation in LPCs exposed to TGF-β. These data indicate the discrepant expression patterns of miRNA between hepatic stem cells and hepatoma-initiating cells, and suggests the potential therapeutic significance of miRNA in HCC targeted therapy. To summarize, our results suggest that TGF-β in cirrhotic liver promotes the neoplastic transformation Small molecule library high throughput of LPCs to hepatic T-ICs and facilitates hepatocarcinogenesis by way of an miR216a/PTEN/Akt-dependent
pathway. These findings not only provide important insight into the molecular mechanism of hepatocarcinogenesis, but also shed new light on the targeting strategy for HCC prevention and therapy. Additional Supporting Information may be found in the online version of this article. “
“This study was designed to demonstrate the safety and efficacy of esomeprazole combined with flupentixol/melitracen for the treatment of gastroesophageal reflux disease (GERD) patients with emotional disorders. Two hundred eighty-nine GERD patients with emotional disorders were divided Decitabine in vivo randomly into two groups: group 1 received esomeprazole only (monotherapy) and group 2 received esomeprazole
and flupentixol/melitracen (combination therapy). The patients’ GERD questionnaire (GerdQ) and hospital anxiety and depression (HAD) scores were obtained before and after treatment. Changes in the scores, rates of symptom remission, and adverse effects were compared between the two groups. After 2 weeks of treatment, the average decrease in GerdQ score in the combination group (4.04 ± 2.34) was significantly greater than that in the monotherapy group (3.34 ± 2.74; P < 0.05). Significant differences between the two groups were also found for changes in HAD anxiety scores (5.45 ± 2.41 vs 3.34 ± 2.43, P < 0.05), Oxalosuccinic acid depression scores (5.47 ± 2.47 vs 3.00 ± 3.28, P < 0.05), and anxiety-depression scores (5.20 ± 2.71 vs 3.60 ± 2.56, P < 0.05). The remission of symptoms (eructation, abdominal pain, anorexia, and other accompanying symptoms) in the combination group was significantly better than that in the monotherapy group, and no significant difference in the incidence of adverse events was observed between the two groups. The combination therapy has better efficacy than the monotherapy in improving the symptoms of gastroesophageal reflux in patients with emotional disorders.