All authors have read and approved the final manuscript.”
“Correction After galley proof of the manuscript, we found three mistakes of the nucleotide positions (G222C, G364A and C520T) and codon numbers (Gly74Arg, Gly122Ser and Thr174Ile) PD0332991 that have to be corrected but it was unable to make any change because the publication of this work is on going [1]. After revision, Table two (Table 1 in this manuscript) and some information in the discussion part were changed. There were only 5 novel mutation types found in this study, consisting of 2 nucleotide substitutions (Leu27Pro and Thr174Ile), 2 nucleotide insertions (G insertion between nucleotide 411 and 412 and GG insertion between nucleotide
520 and 521), and 1 nonsense mutation at Glu127. Table click here 1 Results of pncA gene
sequencing of 150 M. tuberculosis clinical isolates. pncA mutation M. tuberculosis strains (no. of isolates) MGIT 960 PZase assay Nucleotide change Amino acid change Susceptible (46) S + wild-type no Susceptible (1) S + T92G Ile31Ser Susceptible (2) R + wild-type wild-type Susceptible (1) R + T92C Ile31Thr MDR-TB (42) S + wild-type wild-type MDR-TB (9) S + T92C Ile31Thr MDR-TB (34) R – A(-11)G (1) no A(-11)C (1) no T56G (1) Leu19Arg T80C (1) Leu27Pro T92G (2) Ile31Ser T104C (1) Leu35Pro T134C (1) Val45Ala G136T (1) Ala46Ser T199C (1) Ser67Pro C211G (8) His71Asp G215A (1) Cys72Tyr G289A (3) Gly97Ser C312G (2) Ser104Arg G322C (1) Gly108Arg G373T (1) Val125Phe G379T (1) Glu 127 Stop G394A (1) Gly132Ser G insertion b/w 411-412 (1) T416G (1) Val 139 Gly C425T (1) Thr 142 Met G436A (1) Ala 146 Thr GG insertion b/w 520-521 (1) C530T (1) Thr 177 Ile MDR-TB (11) R + wild-type no MDR-TB (4) R + T92C (3) Ile31Thr T92G (1) Ile31Ser We regret any inconvenience that the mistake might have caused. We wish to thank Dr. Claudio Köser, Department of Genetics, University of Cambridge,
Teicoplanin for bringing this matter to our attention. References 1. Jonmalung J, Prammananan T, Leechawengwongs M, Chaiprasert A: Surveillance of pyrazinamide susceptibility among multidrug-resistant Mycobacterium tuberculosis isolates from Siriraj Hospital, Thailand. BMC Microbiology 2010, 10:223.PubMedCrossRef”
“Background The use of contact lenses (CLs) is a major risk factor for the development of microbial keratitis [1–3]. Whilst Gram-negative bacteria, particularly P. aeruginosa, are commonly associated with the condition, within the last four years, two notable outbreaks of CL-associated infectious keratitis have occurred, which were caused by the normally uncommon agents, Fusarium (2006 in Singapore, Hong Kong and the USA) and Acanthamoeba (2007 in USA). These infections were associated with the use of the CL care solutions “”ReNu® with MoistureLoc®”" and “”Complete® MoisturePlus™”", respectively [4].