(B) The killing domain of CaroS2K (Asp677 to carboxyl terminus) h

(B) The killing domain of CaroS2K (Asp677 to carboxyl terminus) has Temsirolimus supplier homology to the minimal tRNase domain of colicin D and klebicin D. (C) The deduced amino acid of immunity protein of CaroS2I has homology

to colicin D and klebicin D. Figure S7. The gene and deduced amino acid sequence of carocin S2 shows in the study. The sequence was truncated form pMS2KI. The underline shows the putative promoter. Figure S8. Schematic representation of thermal asymmetric interlaced PCR (TAIL-PCR) were manipulated according to the method of Liu and Whittier, but the annealing temperature was decreased from 63℃ to 60℃ for specific primers [37, 23]. Amplifying the unknown DNA fragment are the specific primers which are complementary to the known sequence (Tn5) and the arbitrary degenerate primers which could be complementary to the opposite unknown site. The specific primers (SP) are PR1, PR2, PR3, PF1, PF2, PF3, and TF1-2S1

to TF1-2A6 primers for opposite direction (Additional file 1, Table mTOR inhibitor S1). In addition, the arbitrary degenerate primers (AD) N1, N2, and N3 were respectively used as simultaneous PCR amplification (see above). (DOC 14 MB) References 1. Pe’rombelon MCM: Potato diseases caused by soft-rot erwinias: an overview of pathogenesis. The role of pectic enzymes in plant pathogenesis. Plant Pathol 2002, 51:1–12.CrossRef 2. Collmer A, Keen NT: The role of pectic enzymes in plant pathogenesis. Annu Rev Phytopathol 1986, 24:383–409.CrossRef 3. Barras F, Van Gijsegem F, Chatterjee AK: Extracellular enzymes and pathogenesis of soft-rot Erwinia . Annu Rev Phytopathol 1994, 32:201–234.CrossRef 4. Eckert JW, Ogawa JM: The Chemical Control of Postharvest Exoribonuclease Diseases: Deciduous Fruits, Berries, Vegetables and Root/Tuber Crops. Annu Rev Phytopathol 1988, 26:433–469.CrossRef 5. Kikumoto T, Kyeremeh AG, Chuang DY, Gunji Y, Takahara Y, Ehara Y: Biological Control of Soft Rot of Chinese Cabbage Using Single and Mixed Treatments of Bacteriocin-producing Avirulent Mutants of Erwinia carotovora subsp. carotovora . J Gen Plant Pathol 2000, 66:264–268.CrossRef 6. Jack RW, Tagg JR, Ray B:

Bacteriocins of Gram-Positive Bacteria. Microbiol Rev 1995, 59:171–200.PubMed 7. Daw MA, Falkiner FR: Bacteriocins: Nature, Function and Structure. Micron 1996, 27:467–479.PubMedCrossRef 8. Cascales E, Buchanan SK, Duche D, Kleanthous C, Lloube’s R, Postle K, Riley M, Slatin S, Cavard D: Colicin Biology. Microbiol Mol Biol Rev 2007, 71:158–229.PubMedCrossRef 9. Boon T: Inactivation of Ribosomes In Vitro by Colicin E3. Proc Natl Acad Sci USA 1971, 68:2421–2425.PubMedCrossRef 10. Mosbahi K, Walker D, James R, Moore GR, Kleanthous C: Global structural rearrangement of the cell penetrating ribonuclease colicin E3 on interaction with phospholipid membranes. Protein Sci 2006, 15:620–627.PubMedCrossRef 11. Senior BW, Holland IB: Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli .

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