Fig. 2 Mean (± standard error of the mean) plasma GLPG0259 Selleck LY2835219 concentrations after once-daily repeated oral dosing in fed healthy subjects: (a) dosing for 5 days (n = 6 per dose group); (b) dosing for 14 days (n = 6 per dose group). After single dosing, Cmax and AUC24h increased proportionally within the 15–100 mg and 30–150 mg dose ranges (table I). A significant dose effect on tmax was observed, with a higher median value observed at the two highest doses. Although no statistical analysis was performed on t1/2,λz, no noticeable difference in this parameter was observed, with a mean value of about 26.0 hours (range 25.5–26.4 hours). GLPG0259 Repeated-Dose Pharmacokinetics (Studies
1 and 2) GLPG0259 Evofosfamide nmr plasma concentration–time data are plotted in figure 2, and the pharmacokinetic parameters are listed in table II. As was already evident from the single-dose pharmacokinetics, GLPG0259 was absorbed slowly, with a trend toward an increase in tmax with increased dosing (table II).
Table II GLPG0259 pharmacokinetic parameters after once-daily repeated oral dosing in fed healthy subjects (n = 6 per dose group) The steady-state GLPG0259 plasma concentration was reached at between 4 and 8 dosing days (figure 2, table III). After the last dose, plasma elimination of GLPG0259 Selleckchem Ruxolitinib over time displayed a monophasic profile, with a t1/2,λz of about 39 hours (range 35.0–41.6 hours). An approximate 2.5-fold increase in AUC24h and Cmax of GLPG0259, similar for all doses, was observed after once-daily dosing, which was consistent with the long GLPG0259 t1/2,λz. After repeated administration, GLPG0259 did not deviate from dose proportionality, with AUC24h
and Cmax increasing in proportion to the dose within SB-3CT the 20–75 mg dose range. Overall, the between-subject variability in AUC24h and Cmax at steady state was low/moderate (between-subject CV range 16–30%) as was the within-subject variability, which was derived from the square root of the mean square error of the ANOVA (the CVs of AUC and Cmax ranged between 9.8% and 20%; data not shown). Table III Trough plasma GLPG0259 concentrations after once-daily repeated oral dosing in fed healthy subjects (n = 6 per dose group) Excretion of unchanged GLPG0259 in urine was rapid, with about 64–88% excreted within the first 12 hours (data not shown). The Ae24h of GLPG0259 represented 4.99% and 10.4% of the dose administered after single and multiple dosing, respectively, of 50 mg of GLPG0259 for 5 days (table II). The increase in the amount of GLPG0259 excreted in urine between the first and last doses mirrored the accumulation of GLPG0259 observed in plasma. As a consequence, the CLR24h remained constant between the first and last doses. At the 20 mg dose, the increase in Ae24h between the first and last doses (from 3.47% to 4.