Lifestyle-related factors Self-reported lifestyle-related factors were measured both at baseline and at 1-year follow-up. Physical activity (PA) was measured in the baseline questionnaire by check details the short version of the international physical activity questionnaire (IPAQ), which assessed vigorous and moderate intensity PA (Craig et al. 2003). The average time spent on PA per day was calculated. Walking was not included in this calculation, since casual walking is regarded a light-intensity activity. For all behaviors, a dichotomous variable was calculated for non-compliance with the national recommendations. For insufficient

moderate PA, a cut-off point of <30 min of PA per day was used, and for insufficient vigorous PA, a cut-off point of <3 times a week vigorous PA. For insufficient fruit and vegetable intake, the cut-off point was <400 g of fruit and vegetables. Fruit and vegetable intake was measured with the nine-item validated Dutch Food Frequency Questionnaire (Bogers et al. 2004). Smoking was defined as current smoking status, and excessive alcohol use as drinking 15 or more glasses of alcohol per week

for women and 22 or more glasses for men. Health indicators Self-reported BIBW2992 in vivo health and body mass index (BMI) were measured at baseline and at 1-year follow-up. The first question of the short form-12 (SF-12) questionnaire was used to measure perceived general health and dichotomized into ‘poor or moderate’ and ‘good to excellent’ (Ware et al. 1996). In the physical health check, height and weight were measured to calculate the body mass index (BMI) and to categorize individuals as normal weight (BMI < 25 kg/m2), overweight (25 ≤ BMI < 30 kg/m2), and obese (BMI ≥ 30 kg/m2). In the first follow-up, weight was self-reported in the questionnaire. Work-related factors The self-reported work-related factors were measured in the baseline questionnaire. Participants were asked to indicate whether their current job is mainly physically or mentally demanding. In addition, Thymidylate synthase specific psychosocial and physical work demands were asked. The following psychosocial factors were measured with an abbreviated version of a validated Dutch questionnaire about psychosocial

job demands on job stress: work demands (6 items, Ralimetinib in vitro Cronbach’s α = 0.82), job control (4 items, Cronbach’s α = 0.89), skill discretion (4 items, Cronbach’s α = 0.78), and support from colleagues (6 items, Cronbach’s α = 0.74) and supervisor (6 items, Cronbach’s α = 0.79) (Van Veldhoven and Meijman 1994). Questions on work demands were related to excessive work, and insufficient time to complete the work. Job control concerned influence on the planning of tasks, and influence on the pace of work. Skill discretion related to creativity, varied work, and required skills and abilities. Support from colleagues and supervisors was measured with questions related to conflicts, understanding, possibility to ask for help and to count on them, and the atmosphere.

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