NS4B strongly bound to STING in both HEK293T cells and Huh7 cells

NS4B strongly bound to STING in both HEK293T cells and Huh7 cells, suggesting specific molecular interactions, whereas NS4B and Cardif did not show any obvious interaction (Fig. 5A,C). Consistent with previous reports, STING and

Cardif showed significant interaction (Fig. 5B,D). Interestingly, those interactions were https://www.selleckchem.com/products/AG-014699.html decreased by coexpression of NS4B, depending on its input amount, and finally blocked completely in both HEK293T and Huh7 cells (Fig. 5B,D). Collectively, the results above demonstrate that NS4B disrupts the interaction between Cardif and STING possibly through competitive binding to STING. We next studied the impact of STING-mediated IFN production and its regulation by NS4B on HCV infection and cellular replication. First, we transfected three STING-targeted

siRNAs into Huh7/Feo cells (Fig. 6A). As shown in Fig. 6B, STING knockdown cells conferred significantly higher permissibility to HCV replication. We next transfected HCV-JFH1 RNA into Huh7 cells that were transiently transfected with NS4B. As shown in Fig. 6C, HCV core protein expression was significantly higher in NS4B-overexpressed cells. Furthermore, HCV replication was increased significantly in Huh7/Feo cells overexpressing NS4B (Fig. 6D). Taken together, the results above demonstrate that STING and NS4B may negatively or positively regulate cellular permissiveness to HCV replication. It has been reported that the N-terminal domain of several forms of flaviviral NS4B shows structural homology with STING.24 We therefore investigated Wnt inhibitor whether the STING homology domain in NS4B is responsible for suppression of IFN-β production. We constructed two truncated NS4B expression plasmids, which covered the N terminus (NS4Bt1-84, amino acids 1 through 84) containing the STING homology domain and the C terminus (NS4Bt85-261, amino acids 85 through 261), respectively (Fig. 7A). Immunoblotting showed that NS4Bt1-84 and NS4Bt85-261 yielded protein bands of ∼9 kDa selleck chemicals and ∼20 kDa, respectively. Aberrant bands in the truncated NS4B may be due to alternative posttranslational processing. HEK293T cells were transfected with ΔRIG-I, Cardif,

or STING, and NS3/4A or the truncated NS4B, along with IFN-β-Fluc plasmid, and a reporter assay was performed. NS4Bt1-84 significantly suppressed RIG-I, Cardif, and STING-induced IFN-β promoter activity, whereas NS4Bt85-261 did not (Fig. 7B). These results suggest that the N-terminal domain of NS4B is responsible for association with STING. Fluorescent microscopy indicated that both NS4Bt1-84 and NS4Bt85-26 colocalized with ER and STING (Fig. 7C). It has been reported that HCV NS3/4A serine protease cleaves Cardif between Cys-508 and His-509, releases Cardif from the mitochondrial membrane, and blocks RIG-I–induced IFN-β production. We next assessed whether NS4B suppresses IFN-β production in the presence of Cardif cleaved by NS3/4A protease (Cardif1-508, Fig. 8A).

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