radiovictrix relative to the type strains selleckchem Alisertib of the other species within the class ��Deinococci��. The tree was inferred from 1,457 aligned characters [3,4] of the 16S rRNA gene sequence under … A representative genomic 16S rRNA sequence of strain RQ-2T was compared using NCBI BLAST under default settings (e.g., considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database [14] and the relative frequencies, weighted by BLAST scores, of taxa and keywords (reduced to their stem [15]) were determined. The five most frequent genera were Deinococcus (84.0%), Truepera (8.0%), Oceanithermus (6.3%), Thiocapsa (0.9%) and Thiobaca (0.8%) (98 hits in total). Regarding the two hits to sequences from members of the species, the average identity within HSPs was 99.
7%, whereas the average coverage by HSPs was 97.6%. Among all other species, the one yielding the highest score was O. profundus, which corresponded to an identity of 87.5% and an HSP coverage of 61.9%. The highest-scoring environmental sequence was “type”:”entrez-nucleotide”,”attrs”:”text”:”EU924247″,”term_id”:”197244344″,”term_text”:”EU924247″EU924247 (‘Microbiology and geochemistry Little Hot Creek hot spring sediment temperature 80 degrees C clone LHC1 L4 D07′), which showed an identity of 96.6% and an HSP coverage of 91.4%. The five most frequent keywords within the labels of environmental samples which yielded hits were ‘rock’ (3.1%), ‘microbi’ (2.9%), ‘skin’ (2.1%), ‘soil’ (1.9%) and ‘air’ (1.6%) (152 hits in total).
The five most frequent keywords within the labels of environmental samples which yielded hits of a higher score than the highest scoring species were ‘rock’ (4.2%), ‘microbi’ (2.7%), ‘air’ (2.4%), ‘soil’ (2.1%) and ‘cabin/commerci’ (2.0%) (60 hits in total), indicating the existence of close relatives of the strain also in less extreme habitats. The non-motile, red-pigmented cells of RQ-24T are spherical and approximately 1.25-2.0 ��m in diameter, forming predominantly pairs or tetrads (Figure 2 and Table 1). Ultrathin sections of the cytoplasm revealed ribosomes, a fibrillar nucleoid and tubular structures of unknown nature, as well as three distinct layers of the cell wall, the innermost layer being thin, the outermost layer being as thick as 20-90 nm, both being electron dense [1].
Strain RQ-24T grows best at around 50��C, but not at 20��C or 60��C, with 1.0% NaCl in Thermus medium or Degryse medium 162. The acceptable salinity range supporting growth Drug_discovery is up to 6.0% NaCl. Optimum pH is 7.5-9.5, with growth detected up to pH 11.2, but no growth was detected at pH 6.0 [1]. Strains RQ-24T and TU-8 are the most alkaliphilic members of the phylum ��Deinococcus/Thermus�� [1]. Both strains are oxidase and catalase positive and use a wide range of carbohydrates, organic acids or amino acids as carbon and energy sources with a respiratory metabolism (Table 1).