The analysis of the data was realized in a semi-quantitative manner, the scores presented a variation from “−” for no labelling to “+, ++ and +++” to less, moderate and intense labellings, respectively. As described in previous studies from our lab,11 and 12
estrous cycle was monitored and OVX/O and OVX/RLX group presented diestrus smear, atrophied uterine horns and lower plasmatic concentration of estradiol. In contrast, the animals submitted to sham surgery presented the four regular stages of the INCB024360 in vitro estrous cycle, and the animals of group OVX/E2 presented enucleated cornified cells. For all experimental groups, positive immunolabelling for OPG and RANKL protein were visualized in cells of connective tissue, osteoblasts around the trabeculae bone and in osteocytes
aprisioned in the bone tissue formed during the alveolar healing process. TRAP protein was observed in osteoclasts present around the alveolar walls and close to the neoformed trabeculae bone. At 7 postoperative days, besides the great amount of haemosiderin, it was observed discrete RANKL immunolabelling in osteoblasts around trabeculae bone and osteocytes of the middle third (Fig. 1). Fibroblasts of the connective tissue presented moderate immunolabelling Sorafenib datasheet of OPG protein (Fig. 2). OVX/O group presented the highest immunolabelling for OPG and RANKL protein than the other groups. TRAP immunolabelling were not visualized in the middle third, only a discrete labelling
in the borders of the dental 3-mercaptopyruvate sulfurtransferase socket with no significant difference between the groups (Fig. 3). At 14 postoperative days, it was observed RANKL immunolabelling (Fig. 1) similar to the previous period of all groups. Sham and OVX/RLX groups showed similar OPG immunolabelling (Fig. 2) compared to the previous analysed period, whilst OVX/O and OVX/E2 showed a decreasing of OPG immunolabelling. No background labelling with haemosiderin was observed which facilitates the visualization of the area. OVX/O group showed intense TRAP immunolabelling, moderate for OVX/E2 group and discrete for sham and OVX/RLX groups (Fig. 3). At 21 postoperative days, OVX/O group showed a decreasing OPG immunolabelling whilst it was increased for OVX/RLX group compared to the previous period (Fig. 2). Additionally, an increasing of RANKL immunolabelling was observed for all experimental groups (Fig. 1). These findings suggest an increasing in the cellular activity of bone remodelling process in order to form bone tissue in the presence of raloxifene. Considering TRAP immunolabelling, OVX/O group showed an intense expression, OVX/E2 group showed a moderate expression whilst sham and OVX/RLX showed a discrete expression, similar to previous analysed period (Fig. 3).