This was due to the immunological reaction of the host oyster causing early histological efforts to be unable to track the cells of the mantle tissue past the grafting process due to cell differentiation (Kawakami, 1952a, Kawakami, 1952b, Herbaut et al., 2000 and Cochennec-Laureau et al., 2010). However, genotyping the pearl sac using microsatellite NVP-BKM120 nmr genetic markers in Pinctada margaritifera recently confirmed that DNA originating from the donor oyster can still be detected in the pearl sac at pearl harvest ( Arnaud-Haond et al., 2007). Also the influence of the donor oyster on pearl phenotypes such as colour has been shown through examination of pearl quality traits produced from xenografting
two distinct coloured pearl producing species. Here, black-coloured
pearls were produced from Pinctada maxima (silver-lip) host oysters seeded with mantle tissue from P. margaritifera (black-lip) donor Selleckchem Dasatinib oysters, a colour not present in P. maxima pearls ( McGinty et al., 2010). Molecular work by McGinty et al. (2011) has also shown through the use of xenografts in these two species, that two shell matrix proteins are expressed only by the donor oyster within the pearl sacs of P. maxima and P. margaritifera. The phenotypic evidence that pearl traits such as nacre colour are related to the donor oyster, and the molecular verification that the donor oyster expresses two shell matrix proteins (N44, N66) within the pearl sac at pearl harvest, demonstrates that the donor oyster cells are not only present throughout the pearl development process, but are also likely to be actively
involved in cultured pearl formation. To fully elucidate the extent to which the donor PAK6 oyster contributes to pearl formation, the origin of more biomineralisation-related genes expressed within the pearl sac needs to be examined. One of the biggest impediments in determining whether biomineralisation genes in the pearl sac are transcriptionally derived from donor or host cells is being able to first identify all biomineralisation-related genes expressed in the pearl sac at pearl harvest. Previous technology applied to examine the expression of biomineralisation-related genes has predominantly relied upon the examination of genes on an individual basis (e.g. through real time PCR) (Wang et al., 2009 and Inoue et al., 2010). Recent developments in high-throughput mRNA sequencing using next-generation sequencing platforms now provide a potential way to simultaneously examine all biomineralisation genes that are being expressed at one time. The second impediment in determining donor or host cell pearl biomineralisation gene activity is being able to discern the origin of gene transcripts. To date there is a lack of data on intra-specific polymorphisms in biomineralisation genes to allow the characterisation of gene products derived from individual oysters that were used as donors or hosts.