transcripts induced by egg laying, as it repre selleck screening library sents a broad snapshot of the transcriptome and of the ac tivity in the different biochemical pathways in elm. We compared Unitrans Inhibitors,Modulators,Libraries distributions and gene ontology terms and identified enzyme differences among the treat ments especially with regard to egg induced changes in transcript abundances. Leaf beetle egg laying increases defense gene transcripts and decreases transcripts for photosynthesis Gene ontology analysis indicated a decrease in the tran scription level for those genes involved in photosynthesis in the egg and MeJA induced plants. Egg laying by herb ivorous insects can cause a reduction in photosynthetic activity, as has been shown for a tree species and a crop plant. Whether transcription of photosynthesis genes in egg free leaf parts is affected by eggs has not been studied so far.
There has been only one previous study showing a reduc tion of transcription Inhibitors,Modulators,Libraries of photosynthesis related genes after egg laying, however, in this study tissue situated directly underneath the egg masses without full access to light had been sampled. In our study, the material sampled for Inhibitors,Modulators,Libraries sequencing included leaf tissue immediately adjacent to the egg laying site as well as that some distance away. The analyzed tissue was not covered by eggs and had full access to light, and thus the response seen in photosynthesis related genes is not just a response to low light. Our results are consistent with that of other studies showing the reduc tion of photosynthesis Inhibitors,Modulators,Libraries related genes after MeJA treatment.
Further it appears that MeJA affected transcript levels in a manner similar to the insect treatments, which has also been observed in several other studies of plant responses to insect feeding damage. The tran scripts of MeJA treated plants showed GO term distri butions similar to the transcripts of EF treated plants. Both egg Batimastat laying and JA treatments induce the indirect defenses of elms by stimulating the emission of volatiles that attract egg parasitoids. Nevertheless, these different experimental treatments induce volatile patterns that differ qualitatively and quantitatively. In contrast, only minor differences in the overall transcript levels were detected between un treated plants and plants with transferred eggs, indicat ing that the experimental imitation of the egg laying event does not cause any wholesale change in transcrip tional levels.
The GO analysis indicated an increase in the number and quantity of expressed genes involved in defense responses for egg induced plants. In a similar way, an in verse selleck catalog correlation between photosynthesis and defense related genes was observed in Arabidopsis thaliana after egg laying by Pieris brassicae, which might indicate a reallocation of resources from primary to secondary metabolism. However, in Brassica oleracea var. gemmi fera, only a few defense genes were found to respond to treatment of leaves with pierid eggs. Induced defense genes encode PR proteins, chitinases,