A final melt at 95°C for 1 min was done prior to a dissociation c

A final melt at 95°C for 1 min was done prior to a dissociation curve click here analysis (55°C to 95°C in 0.5°C steps for 10 s increments). Fluorescence signals were measured every cycle at the end of the annealing step and continuously during the dissociation curve analysis. The resulting data were analyzed using iQ5 optical system software (Bio-Rad). All reactions were performed in duplicate (within the assay) and each assay was performed twice, resulting in four evaluations of each sample. Statistical Analysis All statistical analyses were done using SPSS software (SPSS Inc., Chicago, IL, USA). Campylobacter and total bacterial count

data was analyzed for significance using the independent sample t-test or the Mann-Whitney U test, as appropriate. Acknowledgements The authors gratefully thank the staff at Prairie Diagnostic Services, Central Animal Veterinary Hospital and find more the dog owners of the city of Saskatoon, SK for their invaluable assistance KPT-330 clinical trial in sample collection, as well as Champika Fernando for assistance with statistical analyses. This study was supported by a Saskatchewan Health Research Foundation (SHRF) Establishment grant to JEH and a SHRF Postdoctoral Fellowship to

BC. Electronic supplementary material Additional file 1: Table S1. Additional information about the dogs from which samples were collected, including breed, age, diet and symptoms (where applicable). Relevant information about the dogs used in this study, with the healthy dog information provided by their owners at time of sample collection and the diarrheic dog information taken from case file information when sample was submitted for testing at Prairie Diagnostic Services. (DOC 154 KB) References 1. WHO: Fact Sheet Phospholipase D1 No. 255: Campylobacter. Geneva: (WHO); 2000. 2. Bowman C, Flint J, Pollari F: Canadian integrated surveillance report: Salmonella , Campylobacter , pathogenic E. coli and

Shigella , from 1996 to 1999. Canada Communicable Dis Report 2003.,29(Suppl 1(1)): i-vi, 1–32. 3. Samuel MC, Vugia DJ, Shallow S, Marcus R, Segler S, McGivern T, Kassenborg H, Reilly K, Kennedy M, Angulo F, et al.: Epidemiology of sporadic Campylobacter infection in the United States and declining trend in incidence, FoodNet 1996–1999. Clin Infect Dis 2004,38(Suppl 3):S165–174.PubMedCrossRef 4. Newell DG: Campylobacter concisus : an emerging pathogen? Eur J Gastroen Hepat 2005,17(10):1013–1014.CrossRef 5. Labarca JA, Sturgeon J, Borenstein L, Salem N, Harvey SM, Lehnkering E, Reporter R, Mascola L: Campylobacter upsaliensis : Another pathogen for consideration in the United States. Clin Infect Dis 2002,34(11):E59–60.PubMedCrossRef 6. Siqueira JF Jr, Rôças IN: Campylobacter gracilis and Campylobacter rectus in primary endodontic infections. Int Endod J 2003,36(3):174–180.PubMedCrossRef 7. de Vries JJ, Arents NL, Manson WL: Campylobacter species isolated from extra-oro-intestinal abscesses: a report of four cases and literature review.

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