Monthly Archives: January 2019

Experiments were performed with 6- to 8-wk-old BALB/c, C.B-17 SCID 41, LTα−/−28, and CXCR5−/− mice 27. C57BL/6 mice were used as controls. BALB/c mice were immunized i.p. with 100 μg of alum-precipitated 2-phenyl-5-oxazolone coupled to the carrier protein chicken serum albumin … Continue reading →

4–7.6)]. Samples were acquired on a FACSCanto, using FACSDiva software (BD Biosciences), and then analysed with FlowJo software version 9.2 (Tree Star, San Carlo, CA). Fluorescence voltages were determined using matched unstained cells. Compensation was carried out with CompBeads (BD … Continue reading →

p.) or 200 pfu LCMV-WE (i.v.). CD8+ T cells were isolated from naïve P14 (Ly5.1+) or IFNAR−/− P14 (Thy1.1+) mice using anti-CD8 beads (Miltenyi Biotech, Germany) and adoptively co-transferred into naive recipient mice. To study the functionality of memory P14 cells, … Continue reading →

LTD4 is known to prime alveolar macrophages to produce mediators such as MIP-1α, TNF and NO when stimulated with LPS [35]. In our study, sCD14 production in PBMC-CD14+ cultures was blocked by the co-incubation of LTD4 with the LTRA Montelukast. … Continue reading →

We aimed at evaluating TLR2 and TLR4 expression on human neutrophils activated with GM-CSF, IL-15, TNF-α or IFN-γ and challenged with a virulent strain of P. brasiliensis (Pb18). Moreover, we asked if these receptors have a role Antiinfection Compound Library on … Continue reading →

B6Idd3 mice (data not shown). Differences in the proliferative status of CD62Lhi- versus CD62Llo-expressing Tanespimycin clinical trial FoxP3+Tregs could explain the distinct FoxP3+Tregs profiles seen in the islets of NOD and NOD.B6Idd3 mice. To investigate this possibility, proliferation of CD62LhiCD4+CD25+FoxP3+ … Continue reading →

Moreover, dNK and endometrial NK cells within the uterus were found to be closely related [58], and CD56bright pNK cells and CD56dim pNK cells from peripheral blood had a closer transcriptional relationship to each other than with CD56bright dNK cells … Continue reading →

, Hercules, CA, USA). The primer pairs utilized for qPCR are shown in Table 1. The data are presented as the mean + SD and are representative of at least two independent experiments that employed at least four mice in each group, unless … Continue reading →

CD11c-DTR (where DTR stands for diphtheria toxin receptor) mice carry a transgene encoding a DTR-GFP fusion ACP-196 chemical structure protein under the control of a murine CD11c promoter [1]. Our results demonstrate a minimal if any effect if mDCs are … Continue reading →

It is noteworthy that the interaction between CpG motif and TLR9 and the resulting response is affected by the structure of CpG DNA or ODN (24). Furthermore, one of the important findings from studies documenting the ability of stimulated PMN … Continue reading →