One sequence from soil R was of non-fungal, unknown eukaryotic origin. From the 115 fungal ribotypes, 42 could be classified to the species level, an additional 24 at least to the genus level, while the remaining 49 fungal sequences could only be classified to the family or higher taxonomic level. Richness ranged from 19 to 34 for detected and from 20.5 to 51.3 for estimated species numbers (Chao2; Chao 1987) per LY294002 sampling site. Coverage of the libraries ranged from 66.3 to 92.8% of estimated species numbers learn more (see Table 1).
As in a few cases sequencing of more than one representative clone from the same RFLP pattern resulted in closely related but dissimilar sequences, the species numbers given here most likely slightly underestimate the true fungal diversity in the investigated soils. UniFrac analysis
could not detect significant differences between the phylogenetic structures of the fungal communities from the herein studied soils. Bonferroni corrected P-values for pairwise comparisons were all above or equal to 0.1. The calculated environmental distances were between 0.43 and 0.60. No clustering of spatially close CP-690550 locations could be found (the distance between sampling sites M and N, P and R respectively R and T is less then 10 km). All five soils are dominated by Ascomycota, which are represented by 77.7 to 88.2% of the clones in the respective libraries, followed by Basidiomycota, which are represented by 7.5 to 21.3% of the clones in the respective libraries (Fig. 1). Other phyla (Chytridiomycota, Blastocladiomycota as well as Mucoromycotina) Nintedanib (BIBF 1120) were only detected occasionally and at low frequencies. No sequences belonging to the Glomeromycota
were found. At all taxonomic levels from phylum to species soil M showed the lowest observed richness (see Fig. 1 and Table 2). Similarly, predicted species richness, several diversity indices (Magurran 2004) and evenness were lowest for soil M (see Table 1). The dominant species in soil M — a species related to Trichocladium asperum — was represented by nearly 30% of all analysed clones (see Table 2). Fig. 1 Relative abundance of fungal groups in arable and grassland soils. Relative abundances at the phylum (or where appropriate alternative taxonomic ranks; left part) and ordinal (right part) level of clones from libraries from arable soils Maissau (M), Niederschleinz (N), Purkersdorf (P) and Tulln (T) and grassland soil Riederberg (R) Table 2 Species list of fungi from arable and grassland soils in Lower Austria Soila Cloneb Acc.No.c Identificationd Order Phy.e RAf COg M NG_M_A03 GU055520 Trichocladium asperum related Sordariales A 29,2 M NG_M_A01 GU055518 Myrothecium sp.