001) when using the Chicago criteria. Inexperienced raters with minimal training performed well with type III; however variability from the ‘gold standard’ was most evident in under diagnosing type I. In contrast, experienced raters were more conservative when diagnosing type III achalasia. 1. Hernandez JC, et al. Am J Gastroenterol 2012; 107: 207–214. selleckchem 2. Bredenoord AJ, et al. Neurogastroenterol Motil 2012; 24: 57–65. MD O’CONNOR,1,2 V HO,1 P MURPHY1 1University of Western
Sydney, Campbelltown, NSW, Australia, 2560, 2Molecular Medicine Research Group, University of Western Sydney, Campbelltown, NSW, Australia, 2560 Introduction: Interstitial cells of Cajal (ICC) play an important role in Daporinad nmr gut motility and cancer development, however, relatively little is known of the molecular mechanisms that drive these processes. To identify novel candidate molecular regulators of ICC biology we developed and applied a novel bioinformatic tool to analyse ICC datasets within the Gene Expression Omnibus (GEO) repository. Materials and methods: An Excel-based macro was developed
that sorts user-downloaded GEO data, containing gene identifiers and associated expression values, to produce a list of genes ranked from highest to lowest expression across multiple samples. To discover novel candidate ICC biology we used the macro, together with a range of other bioinformatic tools, to analyse the GEO ICC dataset, GSE7809. Results: Bioinformatic analysis of gene lists generated via the macro predicts EGR, ETS, and ATF transcription factor families as novel regulators of ICC gene expression (Figure 1). These results have significant implications for the control of ICC behaviour in normal and disease states. P SUNDARALINGAM, V HO Department of Gastroenterology, Campbelltown Hospital, NSW University of Western Sydney, Campbelltown, NSW
Background: Patients with severe and refractory reflux symptoms can present as a significant management issue. A hypotensive lower esophageal sphincter (LES) and anatomic disruption of the gastro-oesophageal junction, often associated with a hiatal this website hernia, are well established pathophysiological associations of gastro-oesophageal reflux disease (GORD). High resolution manometry can provide valuable data regarding oesophageal pathology. We assessed its utility in the evaluation of patients with severe and/or refractory symptoms of GORD at an outer metropolitan teaching hospital in NSW. Methods: We identified all patients who underwent BOTH 24 hr pH monitoring and high resolution manometry for the evaluation of symptoms compatible with GORD at Campbelltown hospital in a retrospective manner using our local motility clinic database. The period reviewed was from the time of the establishment of the database in September 2011 till April 2013.