59, 95% confidence interval: 1.00-6.68, p < 0.05). No significant relationship was observed between m
region genotypes and pre-EPIYA deletion types (Table 2). H. pylori genotypes and histology We examined whether the vacA genotypes and the cagA pre-EPIYA types were related to histological score. The five cagA-negative cases were excluded from histological analysis. Univariate analysis showed that the antral mononuclear cell infiltration scores were significantly higher in tissue infected with Vietnamese or East Asian pre-EPIYA types www.selleckchem.com/products/px-478-2hcl.html than in those infected with the Western type (Table 3). The East Asian cagA repeat type was highly associated with severe mononuclear cell infiltration (p < 0.01) and the type III cag right-end junction was associated with mild neutrophil infiltration (p < 0.01) (Tables 3 and 4). In contrast, there was no relationship between vacA middle-region genotypes and histological score (data
not shown). There was no significant relationship between cagA genotypes and scores for atrophy and intestinal metaplasia (data not shown). Table 3 Histological scores of mononuclear cell infiltration in patients with chronic gastritis infected with H. pylori Selleckchem Captisol strains of different cagA genotypesin the antrum. Mononuclear cell infiltration pre-EPIYA typing EPIYA repeat typing cag right-end junction typing Grade Vietnamese East Asian Western East Asian Western I II III none 0 0 0 0 0 0 0 0 mild 24 5 5 31 4 5 28 2 moderate 52 8 0 61 0 4 55 2 severe 4 0 0 4 0 0 4 0 p-value * p ** p *** p N.S. Mann-Whitney rank sum test. N.S.: not significant. H 89 cell line * p < 0.01; ** p < 0.05; vs. Western type *** p < 0.01 Table 4 Histological scores of neutrophil infiltration in patients
with chronic gastritis infected with H. pylori strains of different cagA genotypes in the antrum. Neutrophil infiltration pre-EPIYA typing EPIYA repeat typing cag right-end junction typing Grade Vietnamese East Asian Western East Asian Western I II III none 4 1 1 7 0 0 4 3 mild 49 9 4 59 4 6 56 1 moderate 26 3 0 29 0 3 26 0 severe 1 0 0 1 0 0 1 0 p-value Rebamipide N.S. N.S. ** p * p Mann-Whitney rank sum test. N.S.: not significant. *p < 0.01; **p < 0.05; vs. type III Multiple linear regression analysis was performed to determine which factor(s) was related to severity of histology. In the antrum, the cag end junction type III was significantly associated with milder neutrophil infiltration (partial regression coefficient [PRC] ± SE = -1.13 ± 0.35 compared with type I, p < 0.001) and more severe intestinal metaplasia (0.61 ± 0.27, p < 0.05) (Table 5). The PRC of -1.13 for the cag end junction type III for neutrophil infiltration suggests that the neutrophil infiltration score associated with cag end junction type III strains would be expected to be 1.12 points lower than with type I strains.