‘Gold Rush’ USA New York D. Rossenberger FR716680 FR716671 FR716662 *128073 *LHY-HNIb-8 *18167 On fruit surface of apple, cv. ‘Fuji’ China Henan H. Li FR716681 FR716672 FR716663 Scleroramularia pomigena *128072 *MA53.5CS3a *16105 On fruit surface of apple, cv. ‘Golden Delicious’ USA Massachusetts A. Tuttle FR716682 FR716673 FR716664 Scleroramularia shaanxiensis *128080 *LHY-mx-3 *18168 On fruit surface of apple, cv. ‘Fuji’ China Shaanxi H. Li FR716683 FR716674 FR716665 Ex-type strains are indicated with an asterisk.
a CBS CBS-KNAW Tucidinostat chemical structure fungal Biodiversity Centre, Utrecht, The Netherlands b CMG Culture collection buy PND-1186 of M. Gleason, housed at Iowa State University, Ames Iowa c CPC Culture collection of P.W. Crous, housed at CBS d ITS Internal transcribed spacers 1 and 2 together with 5.8S nrDNA e LSU 28S nrDNA f TEF partial translation elongation factor 1-alpha To clarify how conidia are produced in this group, and add information pertaining to the nature
of their conidial hila and conidiogenous scars, scanning electron micrographs (SEM) were taken of two isolates from China. After cultures were maintained on PDA for 1 mo in darkness at room temperature, sterile cover slips with attached hyphae were fixed in 3% glutaraldehyde and 1% osmium tetroxide in 0.1 M cacodylate buffer (pH 6.8), followed by a series of ethanol rinses; then the hyphae were dehydrated in MK-8931 mouse a critical point drier, sputter-coated with gold, and examined under a scanning electron microscope (Joel JSM 6360LV) at accelerating voltages of 15 and 25 KV (Zhang et al. 2009). DNA isolation, amplification
and phylogeny Genomic DNA was isolated from fungal mycelium grown on MEA, using the UltraClean™ Microbial DNA Isolation Kit (Mo Bio Laboratories, Inc., Solana Beach, CA, CYTH4 U.S.A.) according to the manufacturer’s protocols. Part of the nuclear rDNA operon spanning the 3′ end of the 18S nrRNA gene (SSU), the first internal transcribed spacer (ITS1), the 5.8S nrRNA gene, the second ITS region (ITS2) and the 5′ end of the 28S nrRNA gene (LSU) was amplified for some isolates as explained in Lombard et al. (2010) and partial translation elongation factor 1-alpha (TEF) gene sequences were determined as described in Bensch et al. (2010). The generated sequences were compared with other fungal DNA sequences from NCBI’s GenBank sequence database using a blastn search. The sequences obtained from GenBank were manually aligned using Sequence Alignment Editor v. 2.0a11 (Rambaut 2002). Phylogenetic analyses of the aligned sequence data were performed using PAUP (Phylogenetic Analysis Using Parsimony) v. 4.0b10 (Swofford 2003). The parsimony analyses were run with alignment gaps treated as a fifth character state and all characters were unordered and of equal weight.