GUS expression was observed at late stage of flower development in styles of florets and selleck chemical in TSTs and guard cells of basal bracts. GUS expression after wounding showed that LIS is involved in plant responsiveness to wounding. Furthermore, the LIS promoter responded to methyl jasmonate (MeJA). These results indicate that the promoter carries a number of cis-acting regulatory elements involved in the tissue-specific expression of LIS and in the response of the plant to wounding and MeJA treatment. Southern blot analysis indicated
that the GUS gene was integrated in the A. annua genome as single or multi copies in different transgenic lines. Promoter activity analysis by qPCR showed that both the wild-type and the recombinant promoter are active in the aerial parts of the plant while only the recombinant promoter was active in roots. Due to the expression in TSTs but not in glandular
trichomes, it may be concluded that US expression will most likely have little or no effect on artemisinin production. (C) 2013 Elsevier GmbH. All rights reserved.”
“Radiation esophagitis is one of the commonest complications of the radiotherapy involving esophagus. It is characterized by diffuse radiotracer uptake in the esophagus on F-18-FDG PET/CT. Thus, it can be often confused with the esophageal malignancy. We present the sequential F-18-FDG PET/CT images of a 45-year-old woman with locally advanced squamous cell carcinoma of the esophagus who developed radiation esophagitis AZD8055 after chemoradiotherapy. It was confused
with the progression of the disease, and the dilemma was CAL 101 resolved by the follow-up PET/CT.”
“Pioneering clinical stem cell research is being performed in the horse, a recipient of cutting edge veterinary medicine as well as a unique animal model, paving the way for human medical applications. Although demonstrable progress has been made on the clinical front, in vitro characterization of equine stem cells is still in comparatively early stages. To translate the promising results of clinical stem cell therapy in the horse, advances must be made in the characterization of equine stem cells. Aiming to improve communication between veterinarians and other natural scientists, this review gives an overview of veterinary “bedside” achievements, focusing on stem cell therapies in equine orthopedics as well as the current state of in vitro characterization of equine multipotent mesenchymal stromal cells (MSCs) and equine embryonic stem cells (ESCs). (C) 2012 International Society for Advancement of Cytometry”
“A series of well-defined thermoresponsive diblock copolymers (PEO45-b-PtNEA(n), n = 22, 44, 63, 91, 172) were prepared by the atom transfer radical polymerization of trans-N-(2-ethoxy-1,3-dioxan-5-yl) acrylamide (tNEA) using a poly(ethylene oxide) (PEO45) macroinitiator.