HnRNP F overexpression attenuates Agt expression in Akita hnRNP F Tg mice. Immunostaining of renal sections, WB of isolated RPTs, and RT qPCR of hnRNP F HA transgene exposed that hnRNP F expression was signicantly greater in RPTCs from hnRNP F Tg and Akita hnRNP F Tg mice in contrast with WT or Akita mice, respectively. In contrast, immunostaining, WB, and RT qPCR exposed signicantly greater Agt expression in RPTs of Akita mice as compared with WT controls and hnRNP F Tg mice but was diminished in Akita hnRNP F Tg mice. The ndings of Agt immunostaining in mouse kidneys have been additional conrmed by immuno staining for Ang II in mouse kidneys, These data demonstrated that hnRNP F overexpression suppresses Agt expression in RPTs of Akita mice. Overexpression of hnRNP F attenuates hypertension and kidney hypertrophy in Akita hnRNP F Tg mice.
Longitudinal SBP measurement showed that SBP was increased in Akita mice than WT or hnRNP F Tg mice from week 10 to 20, on the other hand, selleckchem Odanacatib there was no signicant big difference in SBP be tween Akita mice and Akita hnRNP F Tg mice detected till week 18, Cross sectional SBP measurement at week twenty revealed that SBP was signicantly elevated in Akita mice compared with WT and hnRNP F Tg mice but signicantly attenuated in Akita hnRNP F Tg mice, demonstrating that hnRNP F overexpression attenuates systemic hypertension. Serum Agt and Ang II ranges didn’t vary signicantly while in the 4 diverse groups of mice studied, In contrast, urinary Agt and Ang II amounts were signicantly increased in Akita mice as compared with WT handle littermates or hnRNP F Tg mice but attenuated in Akita hnRNP F Tg mice, Moreover, Agt and Ang II amounts in complete kidney extracts have been signicantly larger in Akita mice than in WT or hnRNP F Tg mice and have been attenuated in Akita hnRNP F Tg mice, supporting the notion that augmented renal Ang II is likely to be accountable, at least in component, to the growth of hypertension in Akita mice.
Kidney dimension and kidney weightbody fat ratio had been signicantly greater in Akita mice as in contrast with WT controls and hnRNP F Tg mice at twenty weeks of age, and these parameters had been signicantly attenuated in Akita hnRNP F Tg mice, WB of p27Kip1 expression showed that p27Kip1 expression was signicantly elevated in RPTs of Akita mice in contrast with WT controls and hnRNP F Tg mice, and it was signicantly attenuated
in Akita hnRNP F Tg mice. These data even more conrmed the kidney hypertrophy in Akita mice and that hnRNP F overex pression could partially attenuate the kidney hypertrophy. In contrast, blood glucose ranges or urinary albumincreati 9 ratio in Akita mice had been signicantly larger than in WT controls and hnRNP F Tg mice and did not normalize in Akita hnRNP F Tg mice, Histologic research.