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“OBJECTIVE: To evaluate the reclosure-promoting capacity of a neural stem cell line (F3) and a human bone marrow stem cell line (B10) injected into the amniotic cavity of spinal open neural tube defects LY2874455 in vivo (ONTDs) of chick embryos of Hamburger and Hamilton stage 18 or 19.
METHODS: Fifteen chick embryos that survived the procedure were obtained for each of 4 groups: untreated control, F3-, B10-,
and HFF-1 (human foreskin fibroblast)-treated groups. Embryos in the control group underwent ONTD surgery but no cell injection.
RESULTS: Compared with the untreated control and HFF-1 groups, the B10 group showed enhanced reclosure at 3, 5, and 7 days after injection, whereas the F3 group did not. B10 cells were not incorporated into reclosed neural tubes but simply covered ONTDs during the process of reclosure. F3 cells did not cover ONTDs. The cell survival of F3 cells exposed to the chick amniotic fluid in vitro for
48 hours was significantly lower than that of B10 cells.
CONCLUSION: The results confirmed that B10 cells enhance reclosure of ONTDs by covering and protecting neural tissues, not by direct cell incorporation. The lack of reclosure capacity in the F3 group may be related to the poor survival of F3 cells in the amniotic fluid.”
“BACKGROUND: The restoration of sensory and motor function in brachial plexus root avulsion patients is a difficult challenge. The central nervous this website system plays an important role in sensory recovery after peripheral nerve injury and repair.
OBJECTIVE: To investigate the sensory restoration
process after surgery at the cortical level in rodent models with a contralateral C7 nerve transfer.
METHODS: Thirty-five male Sprague-Dawley rats were used in this experiment, and both behavioral www.selleck.cn/products/LY2603618-IC-83.html tests and somatosensory evoked potentials were used to investigate the sensory function recovery of the injured forepaws and the cortical reorganization in the rats postoperatively.
RESULTS: The results demonstrated a dynamic change in the ipsilateral somatosensory cortex, both in the shape and location, where overlapping sensory cortical representations of the healthy and injured forepaws were observed consistently. Behavioral tests show that the sensation first occurred only in the healthy forepaw and later in both when stimulating the injured one, which suggested a tendency of the sensation function to recover in the injured forepaws of the rats as time progressed.
CONCLUSION: The cortical reorganization occurred only in the ipsilateral hemisphere, which is different from the motor cortex reorganization using the same model as that described in a previous study.