Of these, almost two thirds had 250 or more hits, but the BLASTX output was limited to a maxi mum of 250 hits per 90e sequence HTS owing to the large number of HSPs reported by BLAST for some of them. The Gene Ontology database was used to computationally annotate all the sequences Inhibitors,Modulators,Libraries by mapping onto them the functional codes already assigned to known proteins from NCBI NR. Many of these sequence hits matched to a short ATP binding domain, in most cases corresponding to proteins of the actins family. Consequently, that functional class, which was also anomalously over represented, was discarded from the total number of annotations for the 90e set, as shown in Table 2. sequences. The BLAST option in the home page menu allows the user to BLAST sequences of interest against the 90, 98, and 90e databases.
Both nucleotide Inhibitors,Modulators,Libraries and protein searches can be performed. Clicking on the Search button brings up a new window displaying a list of hits. When a score value is selected, the alignment between the query sequence and the Smed454 hit is shown. The site also offers the option of downloading Smed454 sequences of interest. The contig or singleton accession number can be browsed directly from the main home page. When the user searches for a specific contig, a new win dow appears showing the alignment of all the sequencing reads assembled in that contig. At the bottom of that win dow, the result of a pre computed BLAST on the contig consensus sequence is displayed. When a contig, singleton or read name is selected, a new window will display Inhibitors,Modulators,Libraries the requested sequence.
All raw and assembled sequence data are available from that web site too. Functional annotation of 90e sequences In order to characterize the gene families that can be found on Smed454, we annotated the Inhibitors,Modulators,Libraries three datasets, we will focus on 90e dataset here. In total, 42. 42% of the Among the most abundant GO annotations at the biolo gical process level, leaving aside metabolism related fea tures, response to stress was found for 1,070 sequences. This finding was expected because the original biological sample was a mixture of intact and regenerat ing planarians, both normal and irradiated. Regulation of biological process was in the same range, with 1,012 sequences. At the GO molecular function level, binding was the most common annotation, although where possible Inhibitors,Modulators,Libraries a more specific annotion was provided by drilling down to the 2nd level child annotations on the GO graph.
It is interesting to find, among others, 3 sele nium binding activities, since it has been reported that selenium may play an important role in cancer preven tion, immune system function, male fertility, cardiovascu lar and muscle disorders, and prevention and control of the ageing process. Finding selenium binding pro teins would be evidence of selleck chemical Rapamycin the presence of selenopro teins, which are thought to be responsible for most of the biomedical effects of selenium across eukaryota.