The alterations in the bone marrow cell type composition SB525334 solubility dmso of mice from the same experiment are presented in Figure 4. The infection of control mice (CP-P-B+ versus CP-P-B-) led to an increase of the segments content (P = 0.0001) and co-administration
of phages (CP-P+B+ group) markedly increased the percentage of myelocytes (P = 0.0016) and metamyelocytes (P = 0.0000). In CP-treated and infected mice (CP+P-B+) there was a deficit of bands and no segments were present, however application of phages in these mice (CP+P+B+ group) led to a significant (a two-fold) mobilization of myelocytes (P = 0.0068) and bands (P = 0.0495). Interestingly, the phages alone (CP-P+B-) increased (P = 0.0000) the content of segments in control, not infected mice (CP-P-B-). Other changes following phage administration were not significant. Figure 4 Effects of A5/L phages on the bone marrow cell composition in cyclophosphamide-treated and S. aureus -infected mice. S – segments, B – bands, Me – metamyelocytes, My – myelocytes, O – other. Mice were given CP
(350 mg/kg b.w.). After four days 1 × 106 A5/L phages and 5 × 106 S. aureus were administered. The bone marrow was isolated on day 0, just before administration of CP (Control) and at 24 h following infection (day Selleckchem NVP-HSP990 5). The results are presented as the mean value of 5 mice per group. Statistics (day 5): Segments: CP-P-B+ vs CP+P-B+ P = 0.0001 (ANOVA; P = 0.0000); Bands: CP-P-B+ vs CP+P-B+ P = 0.0009; CP+P-B+ vs CP+P+B+ P = 0.0495 (ANOVA; P = 0.0000); Metamyelocytes: CP-P-B+ vs CP-P+B+ P = 0.0003 (ANOVA; Idoxuridine P = 0.0000); Myelocytes: CP+P-B+ vs CP+P+B+ P = 0.0062 (ANOVA; P = 0.0000); Other: CP-P-B+ vs CP+P-B+ P = 0.0003 (ANOVA;P = 0.0000). Statistics (day 0 vs day 5): Segments: CP-P-B- vs CP-P-B+ P = 0.0001; CP-P-B- vs CP-P+B- P = 0.0000 (ANOVA); Metamyelocytes:
CP-P-B- vs CP-P+B+ P = 0.0000 (ANOVA); Myelocytes: CP-P-B- vs CP-P+B+ P = 0.0016 (ANOVA). Effects of the phages on generation of the humoral response to S. aureus and to sheep erythrocytes A possibility existed that phages, beside their direct, protective role during infection, may stimulate generation of specific immune response against bacteria. Figure 5 shows the effects of phage administration on the agglutinin level in mouse sera taken 21 days following intraperitoneal immunization of mice with 5 × 106 of S. aureus (for details see Materials and Methods). The results revealed a strong up-regulation (P = 0.0001) of anti-S. aureus agglutinin titer in CP and phage-treated mice (CP+P+B+) in comparison with a respective control (CP-treated mice) (CP+P-B+ group). The analogous effect of phages in mice not treated with CP was minor (CP-P+B+ versus CP-P-B+ group). The phages also enhanced (not significantly), the titer of hemagglutinins to SRBC in CP-treated and infected mice (data not shown). Figure 5 Enhancing effect of A5/L phages on S. aureus -specific antibodies in cyclophosphamide-treated and infected mice. Mice were given CP (200 mg/kg b.