To date, miR 146a has been found in association with autoimmune diseases such as Sj?grens syndrome, psoriasis, and rheumatoid arthritis. Tang et al. reported that miR 146a was under expressed in peripheral blood mononuclear www.selleckchem.com/products/Trichostatin-A.html cells of Chinese SLE patients. miR 146a was significantly lower in pa tients with active SLE with proteinuria compared to those with inactive SLE. Additionally, SLE patients displayed an inverse correlation between miR 146a Inhibitors,Modulators,Libraries expression and IFN score. Tang et al. also demonstrated that reduction of miR 146a may enhance the signaling due to elevated levels of STAT1 and IRF5 which leads to increased produc tion of IFN. The reduced levels of miR 146a observed in Chinese SLE patients could potentially explain elevation of IFN by loss of regulation of STAT1 expression.
Our present study evaluates the interaction among STAT1, ADAR, CCL2, CXCL10, and miR 146a in SLE patients and healthy controls, demonstrating that all ex cept for miR 146a correlate with IFN score in both SLE patients and healthy donors. Inhibitors,Modulators,Libraries Methods Healthy donors and SLE patients demographic data Whole blood was collected from a total of 103 SLE pa tients and 65 healthy controls enrolled in the University of Florida Center for Autoimmune Diseases registry from 2008 to Inhibitors,Modulators,Libraries 2011. Healthy donors were selected based on no history of autoimmune disease, and all SLE patients satisfied the American College of Rheumatol ogy criteria. Healthy donors only visited the clinic once, therefore, they represent a single visit. There were a total of 180 SLE visits with sequential samples collected in 60 SLE patients.
SLE pa tients and healthy controls were segregated by ethnic profile. All human blood samples were ob tained from Inhibitors,Modulators,Libraries enrolled individuals with the approval of the institutional review board at the University of Florida. This study meets and is in compliance with all ethical standards in medicine and informed consent was 1 obtained from all patients according to the Declaration of Helsinki. Inhibitors,Modulators,Libraries Leukocytes and RNA purification Peripheral blood leukocytes were collected from whole blood using Ambion LeukoLOCK kit. LeukoLOCK filters were washed twice with 3 ml of PBS and stabilized with 3 ml of RNAlater solution. Stabi lized filters were stored for a minimum of 24 h at ?80 C be fore collecting total RNA. Total RNA, including small RNAs, was collected using the Alternative Protocol for the extraction of RNA from cells captured on LeukoLOCK filters using TRI reagent. mRNA and microRNA quantitative RT PCR OAS1, MX1, LY6E, STAT1, CCL2, CXCL10, ADAR, TNF, and pri miR 146a levels were analyzed by TaqMan mRNA assay primers. they mRNA qRT PCR was performed as a duplex with 18S rRNA assayed as the normalizer.