5 mM ATP, 0.3 mM GTP, 10 mM HEPES, 5 mM glucose, 2 mM MgCl2, and Dasatinib 1 mM EGTA (pH 7.3). Pipette resistances ranged 3–13 MΩ. Series access resistance ranged from ≈7 to 35 MΩ and was monitored for consistency. Signals were recorded using a patch-clamp amplifier (PC505B, Warner Instruments) and
digitized with a BNC-2111 A/D board (National Instruments, Austin, TX) using custom-written software in Igor Pro (WaveMetrics, Lake Oswego, OR). Signals were amplified, sampled at 10 kHz, filtered to 2 or 5 kHz, and analyzed using custom routines in Igor Pro. TTX, APV, and picrotoxin were all from Tocris Bioscience (Ellisville, MO). GluN2B-siRNA used in these studies was previously described (Hall et al., 2007). All recordings were performed on pyramidal neurons. In slice recordings, layer II pyramidal neurons were targeted for recording, and slices of cortex were within two sections (700 μm) of the primary somatosensory cortex, as evident by the presence of barrel structures, observable in live differential interference contrast images. Synaptic responses were evoked using concentric bipolar stimulating
electrodes placed in layer IV (Frederick Haer, Bowdoin, ME). Current was evoked with a stimulus isolation unit (Cygnus Technology, Delaware Water Gap, PA) controlled by a PG4000A digital stimulator (Cygnus Technology). For local NMDA stimulation (Figures 2 and S2D), glass electrodes of similar resistance (≈5 MΩ) were filled with agonist (NMDA and D-serine) in artificial cerebral spinal fluid, to which we applied short (10–60 ms), repetitive (10 s Doxorubicin intervals) pressure stimulation (18 psi), using a Picospritzer II (General Valve, Fairfield, NJ). All of the following antibodies were used at 1:1,000 dilution: anti-CaMKII (Abcam), anti-phosphoCaMKII (Abcam), anti-GluN2A (Millipore), and anti-GluN2B (University of California, Davis/National Institutes of Health NeuroMab Facility clone number 59/20). Surface staining was performed
as previously described (Hall et al., 2007). All behavioral testing was conducted at the beginning of the animals’ 12 hr dark cycle under low ambient light. Detailed methods for these tests are presented in the Supplemental Experimental Procedures. Statistical significance was confirmed by t test comparison of mean values obtained from each genotype (or Resminostat experimental condition) compared to control. All data (text and figures) are presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. We thank R. Huganir (Johns Hopkins University) for GluN2A cDNA, A. Barria (University of Washington) for CaMKII RSQD cDNA, G. Rumbaugh (Scripps Research Institute Florida) for SynGAP cDNA, G. Patrick (University of California, San Diego) for T286D and T286A CaMKII cDNAs, and G. Westbrook (Oregon Health and Science University Vollum Institute) for GluN2B KO mice. The GluN2B conditional KO mice were generated by the Integrated Neuroscience Initiative on Alcoholism (INIA-Stress award AA13514 to E.D.).