Glucocorticoid mediated apoptosis is cell variety precise To find

Glucocorticoid mediated apoptosis is cell form exact To determine the apoptotic impact that glucocorticoids have on diverse cell lines, we taken care of CEM C7 14, CEM C1 15, A549, and HeLa cell lines with dexametha sone and performed flow cytometry, The lymphoblastic leukaemia CEM C7 selelck kinase inhibitor 14 cells are sensitive to glucocorticoid induced apoptosis, whereas the CEM C1 15 cell line is known as a chosen in vitro subclone within the CEM C7 14 resistant to GC mediated apoptosis, Despite the fact that A549 lung carcinoma cells are already extensively studied with respect to GR, inconclusive results are actually reported when it comes to their response to glucocorticoid induced apoptosis, although former reports have shown that ectopic GR expression induces apoptosis in these cells, In CEM C1 15 and A549 the percentage of cells in the sub G1 phase was mostly under 10%, in addition to a slight reduce in S phase on the cell cycle 48 h immediately after dexamethasone therapy was observed, From the case of CEM C7 14 cells, prolonged hormone treatment method resulted in the boost of the apoptotic population and concomitant decrease during the quantity of cells entering the S phase with the cell cycle, The impact of phosphorylation to the apoptotic profile of CEM C7 14 cells was investigated by treating UV irra diated or not irradiated cells with dexamethasone to the indicated occasions and subjecting them to FACS analy sis.
As proven in Figure 8A the longer treatment NSC-74859 with dexamethasone increased the Sub G1 population from 21% in 0 h to 56% in 48 h dexamethasone handled cells.